Thursday, March 14, 2013
Wednesday, August 22, 2012
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Blood Red Lake In South Of France Caused By High Salt Concentration (PICTURES)
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The stunning images were captured by 52-year-old Russian photographer Sam Dobson, who was driving through the area when he came across the unusual lakes.
The above Data copied by original source of
The Huffington Post UK | Posted: 14/08/2012 13:41 Updated: 14/08/2012 15:10
Monday, June 4, 2012
Research
Cylindrotheca diatom
Cylindrotheca Diatoms Researched as EPA Source
May 27, 2012
AlgaeIndustryMagazine.com
AlgaeIndustryMagazine.com
recently published paper on diatoms, based on work by M. Thomas Kiran, Chief Technology Office of Kadambari Consultants Pvt Ltd, considers the potential for Cylindrotheca as a potential source for eicosapentaenoic acid, or EPA Omega-3 fatty acid.
Cylindrotheca, an epipelic benthic pennate diatom, was shown to hold promise as a nutraceutical source, and may be useful for aquaculture. Experiments were done on two Cylindrotheca species: Cylindrotheca fusiformis (UTEX 2084) and C. closterium, which was isolated from seawater collected offshore from Visakhapatnam, India. C. closterium was identified through microscopy and rDNA typing.
Type and concentration of nutrient components in the culture medium that promoted best growth and highest lipid accumulation were identified. Lipid content was gravimetrically estimated. For relative comparison of the effects of different culture media on lipid content, the researchers made estimations through rapid in situ screening method using Nile red staining and spectrofluorimetry.
The fatty acid profile of lipid was obtained through gas chromatography-mass spectroscopy. Nualgi, a commercially available micronutrient ready-mix with elements adsorbed as nanoparticles on a modified silica sol, was found to significantly boost growth in both Cylindrotheca species when used in lieu of a conventional micronutrient mix prepared from eight compounds.
Among the three nitrogen sources tested – sodium nitrate (NaNO3), urea, and ammonium chloride (NH4Cl) – best growth of C. fusiformis occurred on nitrate and urea, while NH4Cl was best for C. closterium. Lipid productivity was much higher in cultures supplied with NH4Cl for both Cylindrotheca species and compensated for lower biomass in C. fusiformis.
Both Cylindrotheca species were lipid rich; lipid constituted 18–27% of dry biomass in the medium with NaNO3. Among total fatty acids, polyunsaturated fatty acids constituted <40%, eicosapentaenoic acid 25%, and arachidonic acid ∼8% and ∼4% in C. fusiformis and C. closterium, respectively. NH4Cl, phosphate, and Nualgi micronutrient ready-mix in concentrations optimal for each strain contribute to a good culture medium for Cylindrotheca.
The research was performed by Keerthi Suman, Thomas Kiran, Uma Koduru Devi, and Nittala S. Sarma, collectively representing the Department of Botany and the Department of Marine Chemistry, School of Chemistry, Andhra University, Visakhapatnam 530003, Andhra Pradesh, India. Their work was published in Botanica Marina journal: http://www.degruyter.com/view/j/botm.ahead-of-print/bot-2011-0076/bot-2011-0076.xml?format=INThttp://www.algaeindustrymagazine.com/cylindrotheca-diatoms-researched-as-epa-source/
Culture medium optimization and lipid profiling of Cylindrotheca, a lipid- and polyunsaturated fatty acid-rich pennate diatom and potential source of eicosapentaenoic acid
1Department of Botany, Andhra University, Visakhapatnam 530003, Andhra Pradesh, India
2Department of Marine Chemistry, School of Chemistry, Andhra University, Visakhapatnam 530003, Andhra Pradesh, India
aThe first two authors made equal contributions to the manuscript.
Citation Information: Botanica Marina. Volume 0, Issue 0, Pages -–-, ISSN (Online) 1437-4323, ISSN (Print) 0006-8055, DOI: 10.1515/bot-2011-0076,
Publication History:
Received: 06/08/2011;
Accepted: 28/03/2012;
Published Online: 21/05/2012Abstract
Cylindrotheca, an epipelic benthic pennate diatom, holds promise as a nutraceutical source and may be useful for aquaculture. Experiments were done on two Cylindrotheca species, Cylindrotheca fusiformis (UTEX 2084) andC. closterium, which was isolated from seawater collected offshore from Visakhapatnam, India. C. closteriumwas identified through microscopy and rDNA typing. Type and concentration of nutrient components in the culture medium that promoted best growth and highest lipid accumulation were identified. Lipid content was gravimetrically estimated. For relative comparison of the effects of different culture media on lipid content, we made estimations through rapid in situ screening method using Nile red staining and spectrofluorimetry. The fatty acid profile of lipid was obtained through gas chromatography-mass spectroscopy. Nualgi, a commercially available micronutrient ready-mix with elements adsorbed as nanoparticles on a modified silica sol, was found to significantly boost growth in bothCylindrotheca species when used in lieu of a conventional micronutrient mix prepared from eight compounds. Among the three nitrogen sources tested – sodium nitrate (NaNO3), urea, and ammonium chloride (NH4Cl) – best growth of C. fusiformis occurred on nitrate and urea, while NH4Cl was best for C. closterium. Lipid productivity was much higher in cultures supplied with NH4Cl for both Cylindrotheca species and compensated for lower biomass in C. fusiformis. Mixotrophy with glycerol or sodium acetate resulted in no significant increase in growth over photoautotrophy. Both Cylindrothecaspecies were lipid rich; lipid constituted 18–27% of dry biomass in the medium with NaNO3. Among total fatty acids, polyunsaturated fatty acids constituted <40%, eicosapentaenoic acid 25%, and arachidonic acid ∼8% and ∼4% in C. fusiformis and C. closterium, respectively. NH4Cl, phosphate, and Nualgi micronutrient ready-mix in concentrations optimal for each strain contribute to a good culture medium for Cylindrotheca.
Wednesday, January 25, 2012
This is the recording of the news which covered the algal research going on in Professor K Uma Devi's Lab, Department of Botany, Andhra University, It covered the importance of algae for the environment and a few achievements of the lab. The research personnel who are seen in the video include Mr. Suman Keerthi (SRF), Professor K Uma Devi and Ms. Swapna Guntupalli (SRF) (in order of appearance)
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